Journal article
smoothened and thickveins regulate Moleskin/Importin 7-mediated MAP kinase signaling in the developing Drosophila eye
Development (Cambridge), v 133(8), pp 1485-1494
Apr 2006
PMID: 16540506
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The
Drosophila
Mitogen Activated Protein Kinase (MAPK) Rolled is a key regulator of developmental signaling, relaying information from the cytoplasm into the nucleus. Cytoplasmic MEK phosphorylates MAPK (pMAPK), which then dimerizes and translocates to the nucleus where it regulates transcription factors. In cell culture, MAPK nuclear translocation directly follows phosphorylation, but in developing tissues pMAPK can be held in the cytoplasm for extended periods (hours). Here, we show that Moleskin antigen (
Drosophila
Importin 7/Msk), a MAPK transport factor, is sequestered apically at a time when lateral inhibition is required for patterning in the developing eye. We suggest that this apical restriction of Msk limits MAPK nuclear translocation and blocks Ras pathway nuclear signaling. Ectopic expression of Msk overcomes this block and disrupts patterning. Additionally, the MAPK cytoplasmic hold is genetically dependent on the presence of Decapentaplegic (Dpp) and Hedgehog receptors.
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Details
- Title
- smoothened and thickveins regulate Moleskin/Importin 7-mediated MAP kinase signaling in the developing Drosophila eye
- Creators
- Alysia D Vrailas - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USADaniel R Marenda - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USASummer E Cook - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USAMaureen A Powers - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USAJames A Lorenzen - Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Harvard Medical School Boston, MA 02114, USALizabeth A Perkins - Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Harvard Medical School Boston, MA 02114, USAKevin Moses - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
- Publication Details
- Development (Cambridge), v 133(8), pp 1485-1494
- Publisher
- Company of Biologists
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000236806900008
- Scopus ID
- 2-s2.0-33646678741
- Other Identifier
- 991014878749404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Developmental Biology