Journal article
α4β1 Integrin Mediates the Recruitment of Immature Dendritic Cells across the Blood-Brain Barrier during Experimental Autoimmune Encephalomyelitis
The Journal of immunology (1950), v 184(12), pp 7196-7206
15 Jun 2010
PMID: 20483748
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Dendritic cells (DCs) within the CNS are recognized to play an important role in the effector phase and propagation of the immune response in experimental autoimmune encephalomyelitis (EAE), a mouse model for multiple sclerosis. However, the mechanisms regulating DC trafficking into the CNS still need to be characterized. In this study, we show by performing intravital fluorescence videomicroscopy of the inflamed spinal cord white-matter microvasculature in SJL mice with EAE that immature, and to a lesser extent, LPS-matured, bone marrow-derived DCs efficiently interact with the CNS endothelium by rolling, capturing, and firm adhesion. Immature but not LPS-matured DCs efficiently migrated across the wall of inflamed parenchymal microvessels into the CNS. Blocking α
4
integrins interfered with the adhesion but not the rolling or capturing of immature and LPS-matured DCs to the CNS microvascular endothelium, inhibiting their migration across the vascular wall. Functional absence of β
1
integrins but not of β
7
integrins or α
4
β
7
integrin similarly reduced the adhesion of immature DCs to the CNS microvascular endothelium, demonstrating that α
4
β
1
but not α
4
β
7
integrin mediates this step of immature DCs interaction with the inflamed blood-brain barrier during EAE. Our study shows that during EAE, especially immature DCs migrate into the CNS, where they may be crucial for the perpetuation of the CNS-targeted autoimmune response. Thus therapeutic targeting of α
4
integrins affects DC trafficking into the CNS and may therefore lead to the resolution of the CNS autoimmune inflammation by reducing the number of CNS professional APCs.
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Details
- Title
- α4β1 Integrin Mediates the Recruitment of Immature Dendritic Cells across the Blood-Brain Barrier during Experimental Autoimmune Encephalomyelitis
- Creators
- Pooja Jain - Department of Microbiology and Immunology and Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA 18902Caroline Coisne - Theodor Kocher Institute, University of Bern, Bern, SwitzerlandGaby Enzmann - Theodor Kocher Institute, University of Bern, Bern, SwitzerlandRobert Rottapel - Ontario Cancer Institute, Toronto Medical Discovery Tower, Toronto, Ontario, CanadaBritta Engelhardt - Theodor Kocher Institute, University of Bern, Bern, Switzerland
- Publication Details
- The Journal of immunology (1950), v 184(12), pp 7196-7206
- Publisher
- American Association of Immunologists (AAI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000278516700070
- Scopus ID
- 2-s2.0-77953642555
- Other Identifier
- 991014877708304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology