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Do 15 Million Cat Neurons Mediate the Memory of a Circle and a Star?
Letter/Communication

Do 15 Million Cat Neurons Mediate the Memory of a Circle and a Star?

Donald L McEachron
Science (American Association for the Advancement of Science), v 238(4833), pp 1587-1587
11 Dec 1987
PMID: 3685997

Abstract

E. R. John et al. (1) propose a method for sequential double-labeling with 2-deoxyglu-cose (2DG) in which 14C and 18F are used. Sequential double-labeling with 2DG involves injecting a bolus of [14C]2DG, applying stimulus A for 45 minutes, injecting a second bolus of 2DG either labeled with 3H or 18F and applying stimulus B for a second 45-minute period. By taking advantage of the physical properties of the radioisotopes, the relative inability of 3H to expose coated x-ray film in the case of tritium or a short half-life in the case of 18F and with the use of image-processing techniques, John et al. ascertains the relative contributions of each form of the labeled 2DG. These experiments offer the potential for determining the effects of two different stimuli in single animal or for using an animal as its own control. The primary assumption is that the original [14C]2DG does not relocate in response to stimulation during the second labeling period. [1st paragraph]

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