Letter/Communication
Reply: TIMP-1 enhancer sequence – real or bacterial?
British journal of cancer, v 89(9), pp 1812-1813
28 Oct 2003
PMID: 14583788
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The characterisation of the TIMP-1 gene was published in Wang et al (1998). The TIMP-1 gene was cloned from PC-3 ML genomic DNA. The Pst1–Xba1 fragment at the 5′end of the Pst1 4.0-kilobase TIMP-1 genomic clone was subcloned into plasmid PUC 19. This fragment was sequenced from both ends on opposite strands with the Sequenase Virgin 2.0 sequencing system (US Biochemical). Each strand was sequenced with both the Klenow fragment and reverse transcriptase by standard dideoxy procedures. Blast analysis of the 5′ promoter region revealed a 97% homology with Homo sapiens TIMP-1 promoter region (chromosome X genomic contig, NT 011568). Blast analysis of the coding region revealed a 97% homology with human TIMP-1 (Docherty et al, 1985) and 96% homology with the coding region of the E. coli TIMP-1. [1st paragraph]
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Details
- Title
- Reply: TIMP-1 enhancer sequence – real or bacterial?
- Creators
- M E Stearns - Drexel UniversityY Hu - Drexel UniversityM Wang - Drexel University
- Publication Details
- British journal of cancer, v 89(9), pp 1812-1813
- Publisher
- Nature Publishing Group
- Number of pages
- 2
- Resource Type
- Letter/Communication
- Language
- English
- Academic Unit
- Decision Sciences (and Management Information Systems); Materials Science and Engineering
- Web of Science ID
- WOS:000186789500035
- Scopus ID
- 2-s2.0-0344009698
- Other Identifier
- 991019167516504721
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- Oncology