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Preprint
Impact of temperature on the affinity of SARS-CoV-2 Spike for ACE2
bioRxiv
09 Jul 2021
PMID: 34268505
Abstract
The seasonal nature in the outbreaks of respiratory viral infections with increased transmission during low temperatures has been well established. The current COVID-19 pandemic makes no exception, and temperature has been suggested to play a role on the viability and transmissibility of SARS-CoV-2. The receptor binding domain (RBD) of the Spike glycoprotein binds to the angiotensin-converting enzyme 2 (ACE2) to initiate viral fusion. Studying the effect of temperature on the receptor-Spike interaction, we observed a significant and stepwise increase in RBD-ACE2 affinity at low temperatures, resulting in slower dissociation kinetics. This translated into enhanced interaction of the full Spike to ACE2 receptor and higher viral attachment at low temperatures. Interestingly, the RBD N501Y mutation, present in emerging variants of concern (VOCs) that are fueling the pandemic worldwide, bypassed this requirement. This data suggests that the acquisition of N501Y reflects an adaptation to warmer climates, a hypothesis that remains to be tested.
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Details
- Title
- Impact of temperature on the affinity of SARS-CoV-2 Spike for ACE2
- Creators
- Jérémie Prévost - University of MontrealJonathan Richard - University of MontrealRomain Gasser - University of MontrealShilei Ding - Centre Hospitalier de l’Université de MontréalClément Fage - Université LavalSai Priya Anand - McGill UniversityDamien Adam - University of MontrealNatasha Gupta Vergara - Drexel UniversityAlexandra Tauzin - University of MontrealMehdi Benlarbi - Centre Hospitalier de l’Université de MontréalShang Yu Gong - McGill UniversityGuillaume Goyette - Centre Hospitalier de l’Université de MontréalAnik Privé - Centre Hospitalier de l’Université de MontréalSandrine Moreira - Laboratoire de Santé Publique du Québec, Institut Nationale de Santé Publique du QuébecHugues Charest - Laboratoire de Santé Publique du Québec, Institut Nationale de Santé Publique du QuébecMichel Roger - University of MontrealWalther Mothes - Yale UniversityMarzena Pazgier - Uniformed Services University of the Health SciencesEmmanuelle BrochieroGuy Boivin - Université LavalCameron F. Abrams - Drexel UniversityArne Schön - Department of Biology The Johns Hopkins UniversityAndrés Finzi - University of Montreal
- Publication Details
- bioRxiv
- Publisher
- Cold Spring Harbor Laboratory
- Resource Type
- Preprint
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Other Identifier
- 991019203706904721