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Preprint
Mating induces ecdysone signaling in the Drosophila testis niche disrupting somatic encystment of germ cells and stem cell cytokinesis
bioRxiv
17 Oct 2023
PMID: 37905121
Abstract
Germline maintenance relies on adult stem cells to continually replenish lost gametes over a lifetime and respond to external cues altering the demands on the tissue. Mating worsens germline homeostasis over time, yet a negative impact on stem cell behavior has not been explored. Using extended live imaging of the
Drosophila
testis stem cell niche, we find that short periods of mating in young males disrupts cytokinesis in germline stem cells (GSCs). This defect leads to failure of abscission, preventing release of differentiating cells from the niche. We find that GSC abscission failure is caused by increased ecdysone hormone signaling induced upon mating, which leads to disrupted somatic encystment of the germline. Abscission failure is rescued by isolating males from females but recurs with resumption of mating. Importantly, reiterative mating also leads to increased GSC loss, requiring increased restoration of stem cells via symmetric renewal and de-differentiation. Together, these results suggest a model whereby acute mating results in hormonal changes that negatively impact GSC cytokinesis but preserves the stem cell population.
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Details
- Title
- Mating induces ecdysone signaling in the Drosophila testis niche disrupting somatic encystment of germ cells and stem cell cytokinesis
- Creators
- Tiffany V. Roach - Drexel UniversityKari F. Lenhart - Drexel University
- Publication Details
- bioRxiv
- Publisher
- Cold Spring Harbor Laboratory
- Resource Type
- Preprint
- Language
- English
- Academic Unit
- Biology
- Other Identifier
- 991021481040504721