Preprint
Properties of rhythmogenic currents in spinal Shox2 interneurons across postnatal development
bioRxiv, v 603(10), pp 3201-3221
15 May 2024
PMID: 39386611
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Locomotor behaviors are performed by organisms throughout life, despite developmental changes in cellular properties, neural connectivity, and biomechanics. The basic rhythmic activity in the central nervous system that underlies locomotion is thought to be generated via a complex balance between network and intrinsic cellular properties. Within mature mammalian spinal locomotor circuitry, we have yet to determine which properties of spinal interneurons (INs) are critical to rhythmogenesis and how they change during development. Here, we combined whole cell patch clamp recordings, immunohistochemistry, and RNAscope targeting lumbar Shox2 INs in mice, which are known to be involved in locomotor rhythm generation. We focused on the properties of putatively rhythmogenic ionic currents and the expression of corresponding ion channels across postnatal time points in mice. We show that subsets of Shox2 INs display voltage-sensitive conductances, in addition to respective ion channels, which may contribute to or shape rhythmic bursting. Persistent inward currents, M-type potassium currents, slow afterhyperpolarization, and T-type calcium currents are enhanced with age. In contrast, the hyperpolarization-activated and A-type potassium currents were either found with low prevalence in subsets of neonatal, juvenile, and adult Shox2 INs or did not developmentally change. We show that Shox2 INs become more electrophysiologically diverse by juvenile and adult ages, when locomotor behavior is weight-bearing. These results suggest a developmental shift in the magnitude of rhythmogenic ionic currents and the expression of corresponding ion channels that may be important for mature, weight-bearing locomotor behavior.
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Details
- Title
- Properties of rhythmogenic currents in spinal Shox2 interneurons across postnatal development
- Creators
- Shayna Singh - Marion Murray Spinal Cord Research Center, Department of Neurobiology and Anatomy, Drexel University College of MedicineLihua Yao - Drexel University, Neurobiology and AnatomyNatalia A. Shevtsova - Drexel University, Neurobiology and AnatomyIlya A. Rybak - Drexel University, Neurobiology and AnatomyKimberly J. Dougherty - Drexel University, Neurobiology and Anatomy
- Publication Details
- bioRxiv, v 603(10), pp 3201-3221
- Publisher
- Cold Spring Harbor Laboratory
- Edition
- 1.1
- Number of pages
- 31
- Resource Type
- Preprint
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; [Retired Faculty]
- Other Identifier
- 991022053613604721
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InCites Highlights
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- Web of Science research areas
- Neurosciences
- Physiology