Journal article
A labeled substrate approach to discovery of biocatalytic reactions: a proof of concept transformation with N-methylindole
Journal of the American Chemical Society, v 134(30), pp 12378-12381
01 Aug 2012
PMID: 22809085
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Biocatalysis has become an important method in the pharmaceutical industry for the incorporation of new functionality in small molecules. Currently this method is limited in the types of reactions that can be carried out and no strategy exists to systematically screen for new biocatalyzed reactions. This study involves the development of a medium throughput screen to identify and optimize new reactions using a series of marine-derived bacterial cell lines, which were screened against several (13)C labeled organic substrates. The reactions were analyzed using (13)C NMR as the primary screening tool. We describe the discovery of a bacterial catalyzed indole oxidation reaction in which complete conversion of (13)C labeled N-methyl indole to 3-hydroxyindole was observed. In addition, the sensitivity of this reaction to dO(2) levels can be exploited to oxidize to either 3-hydroxyindole or 2-oxoindole. This new platform sets up an important tool for the discovery of new organic transformations using an extensive library of marine bacteria.
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Details
- Title
- A labeled substrate approach to discovery of biocatalytic reactions: a proof of concept transformation with N-methylindole
- Creators
- Jamie L Rogers - The University of Texas Southwestern Medical CenterJohn B MacMillan - The University of Texas Southwestern Medical Center
- Publication Details
- Journal of the American Chemical Society, v 134(30), pp 12378-12381
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000306942600014
- Scopus ID
- 2-s2.0-84864424508
- Other Identifier
- 991021229908604721
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- Web of Science research areas
- Chemistry, Multidisciplinary