Journal article
Angioneural Crosstalk in Scaffolds with Oriented Microchannels for Regenerative Spinal Cord Injury Repair
Journal of molecular neuroscience, v 49(2), pp 334-346
01 Feb 2013
PMID: 22878912
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The aim of our work is to utilize the crosstalk between the vascular and the neuronal system to enhance directed neuritogenesis in uniaxial guidance scaffolds for the repair of spinal cord injury. In this study, we describe a method for angioneural regenerative engineering, i.e., for generating biodegradable scaffolds, produced by a combination of controlled freezing (freeze-casting) and lyophilization, which contain longitudinally oriented channels, and provide uniaxial directionality to support and guide neuritogenesis from neuronal cells in the presence of endothelial cells. The optimized scaffolds, composed of 2.5 % gelatin and 1 % genipin crosslinked, were characterized by an elastic modulus of similar to 51 kPa and longitudinal channels of similar to 50 mu m diameter. The scaffolds support the growth of endothelial cells, undifferentiated or NGF-differentiated PC12 cells, and primary cultures of fetal chick forebrain neurons. The angioneural crosstalk, as generated by first forming endothelial cell monolayers in the scaffolds followed by injection of neuronal cells, leads to the outgrowth of long aligned neurites in the PC12/endothelial cell co-cultures also in the absence of exogenously added nerve growth factor. Neuritogenesis was not observed in the scaffolds in the absence of the endothelial cells. This methodology is a promising approach for neural tissue engineering and may be applicable for regenerative spinal cord injury repair.
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Details
- Title
- Angioneural Crosstalk in Scaffolds with Oriented Microchannels for Regenerative Spinal Cord Injury Repair
- Creators
- Aybike Saglam - Drexel UniversityAnat Perets - Drexel UniversityAdam Charles Canver - Drexel UniversityHo-Lung Li - Drexel UniversityKatherine Kollins - Drexel UniversityGadi Cohen - Hebrew University of JerusalemItzhak Fischer - Drexel UniversityPhilip Lazarovici - Drexel UniversityPeter I. Lelkes - Drexel University
- Publication Details
- Journal of molecular neuroscience, v 49(2), pp 334-346
- Publisher
- Humana Press Inc
- Number of pages
- 13
- Grant note
- Louis and Bessie Stein Foundation Dr. Adolf and Klara Brettler Center for Research in Molecular Pharmacology and Therapeutics at the Hebrew University of Jerusalem, Israel Israel Ministry of Science and Technology; Ministry of Science, Technology and Space (MOST), Israel Craig H. Neilson Foundation David R. Bloom Center for Pharmacy
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000313519900012
- Scopus ID
- 2-s2.0-84875732263
- Other Identifier
- 991019168472404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences