Journal article
Application of recombinant adenovirus for in vivo gene delivery to spinal cord
Brain research, v 768(1), pp 19-29
1997
PMID: 9369296
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
One strategy for treating spinal cord injury is to supply damaged neurons with the appropriate neurotrophins either by direct delivery or by transfer of the corresponding genes using viral vectors. Here we report the feasibility of using recombinant adenovirus for in vivo gene transfer in spinal cord. After injection of a recombinant adenovirus carrying a
β-galactosidase (
β-gal) reporter gene into the mid-thoracic spinal cord of adult rats, transgene expression occurred not only in several types of cells around the injection site but also in neurons whose axons project to this region from rostral or caudal to the injection site. Among labeled neurons were those of the red nucleus, the vestibular nuclei, reticular formation, locus coeruleus, and Clarke's nucleus. A non-specific immune reaction, which could be blocked by immunosuppression with Cyclosporin A, reduced the number of transduced cells surviving at the injection site by 1 month. In neurons away from the injection site, where the immune response was minimal, transgene expression lasted for at least 2 months. These results support the idea that recombinant adenovirus can be used in the spinal cord for in vivo delivery of therapeutic genes important for supporting neuron survival and axon regeneration.
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Details
- Title
- Application of recombinant adenovirus for in vivo gene delivery to spinal cord
- Creators
- Yi Liu - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USAB.Timothy Himes - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USAJon Moul - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USAWenlin Huang - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USAStella Y Chow - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USAAlan Tessler - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USAItzhak Fischer - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, 3200 Henry Avenue, Philadelphia, PA 19129, USA
- Publication Details
- Brain research, v 768(1), pp 19-29
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1997YB44200003
- Scopus ID
- 2-s2.0-0030862006
- Other Identifier
- 991014878245304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences