Journal article
Carbon monoxide releasing molecule-2 improves coagulation in patient plasma in vitro following cardiopulmonary bypass
Blood coagulation & fibrinolysis, v 22(5), pp 362-368
01 Jul 2011
PMID: 21346555
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The objective of the present study was to determine if a new procoagulant molecule, carbon monoxide releasing molecule (tricarbonyldichlororuthenium (II) dimer; CORM-2) would improve coagulation following cardiopulmonary bypass (CPB). Plasma was obtained from patients undergoing elective cardiac surgery requiring CPB. Whole blood was collected and anticoagulated with sodium citrate after induction of anesthesia and again after CPB and heparin neutralization with protamine. Blood samples were centrifuged for 15 min, with plasma collected and stored at -80 degrees C prior to analysis. Samples were subsequently exposed to 0 or 100 mu mol/l CORM-2, with coagulation activated with tissue factor. Data were collected with thrombelastography until clot strength stabilized. Patients underwent CPB for 133 +/- 61 min (mean +/- SD). The velocity of thrombus formation was significantly decreased (52%) by CPB, as was clot strength (53%). Addition of CORM-2 to plasma samples obtained after CPB significantly increased the velocity of clot formation (75%) and strength (52%) compared to matched unexposed samples. The lesion of plasmatic coagulation associated with CPB was significantly improved in vitro by addition of CORM-2. If preclinical assessments of efficacy and safety of CORM-2 are favorable, future clinical trials involving CORM-2 or other CORMs as a hemostatic intervention in the setting of CPB are justified. Blood Coagul Fibrinolysis 22: 362-368 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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Details
- Title
- Carbon monoxide releasing molecule-2 improves coagulation in patient plasma in vitro following cardiopulmonary bypass
- Creators
- S. Nini Malayaman - Drexel UniversityJohn W. C. Entwistle - Drexel UniversityPercy Boateng - Drexel UniversityAndrew S. Wechsler - Drexel UniversityJoshua M. Persaud - Drexel UniversityJack B. Cohen - Drexel UniversityJames K. Kirklin - University of Alabama at BirminghamVance G. Nielsen - Drexel University
- Publication Details
- Blood coagulation & fibrinolysis, v 22(5), pp 362-368
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 7
- Grant note
- Drexel University College of Medicine Department of Anesthesiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Anesthesiology and Perioperative Medicine; [Retired Faculty]
- Web of Science ID
- WOS:000292506600002
- Scopus ID
- 2-s2.0-79960699795
- Other Identifier
- 991019168795804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Hematology