Journal article
Characterization and intraspinal grafting of EGF/bFGF-dependent neurospheres derived from embryonic rat spinal cord
Brain research, v 874(2)
2000
PMID: 10960593
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Recent advances in the isolation and characterization of neural precursor cells suggest that they have properties that would make them useful transplants for the treatment of central nervous system disorders. We demonstrate here that spinal cord cells isolated from embryonic day 14 Sprague–Dawley and Fischer 344 rats possess characteristics of precursor cells. They proliferate as undifferentiated neurospheres in the presence of EGF and bFGF and can be maintained in vitro or frozen, expanded and induced to differentiate into both neurons and glia. Exposure of these cells to serum in the absence of EGF and bFGF promotes differentiation into astrocytes; treatment with retinoic acid promotes differentiation into neurons. Spinal cord cells labeled with a nuclear dye or a recombinant adenovirus vector carrying the lacZ gene survive grafting into the injured spinal cord of immunosuppressed Sprague–Dawley rats and non-immunosuppressed Fischer 344 rats for up to 4 months following transplantation. In the presence of exogenously supplied BDNF, the grafted cells differentiate into both neurons and glia. These spinal cord cell grafts are permissive for growth by several populations of host axons, especially when combined with exogenous BDNF administration, as demonstrated by penetration into the graft of axons immunopositive for 5-HT and CGRP. Thus, precursor cells isolated from the embryonic spinal cord of rats, expanded in culture and genetically modified, are a promising type of transplant for repair of the injured spinal cord.
Metrics
Details
- Title
- Characterization and intraspinal grafting of EGF/bFGF-dependent neurospheres derived from embryonic rat spinal cord
- Creators
- Stella Y Chow - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USAJon Moul - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USAChris A Tobias - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USAB.Timothy Himes - Department of Veteran Affairs Medical Center, Philadelphia, PA 19104, USAYi Liu - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USAMaria Obrocka - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USALisa Hodge - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USAAlan Tessler - Department of Veteran Affairs Medical Center, Philadelphia, PA 19104, USAItzhak Fischer - Department of Neurobiology and Anatomy, MCP Hahnemann University, 3200 Henry Avenue, Philadelphia, PA 19129, USA
- Publication Details
- Brain research, v 874(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000089107800001
- Scopus ID
- 2-s2.0-0034714306
- Other Identifier
- 991014878046204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences