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Journal article
Cloning and Expression of a Second Photoreceptor-Specific Membrane Retina Guanylyl Cyclase (RetGC), RetGC-2
Proceedings of the National Academy of Sciences - PNAS, v 92(12), pp 5535-5539
06 Jun 1995
PMID: 7777544
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Abstract
One of the membrane guanylyl cyclases (GCs), RetGC, is expressed predominantly in photoreceptors. No extracellular ligand has been described for RetGC, but it is sensitive to activation by a soluble 24-kDa protein (p24) and is inhibited by Ca2+. This enzyme is, therefore, thought to play a role in resynthesizing cGMP for photoreceptor recovery or adaptation. By screening a human retinal cDNA library at low stringency with the cytoplasmic domains from four cyclases, we cloned cDNAs encoding a membrane CG that is most closely related to RetGC. We have named this GC RetGC-2, and now term the initially described RetGC RetGC-1. By in situ hybridization, mRNA encoding RetGC-2 is found only in the outer nuclear layer and inner segments of photoreceptor cells. By using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2 can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments. Membrane GC activity of recombinant RetGC-2 expressed in human embryonic kidney 293 cells is stimulated by the activator p24 and is inhibited by Ca2+with an EC50value of 50-100 nM. Our data reveal a previously unappreciated diversity of photoreceptor GCs.
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Details
- Title
- Cloning and Expression of a Second Photoreceptor-Specific Membrane Retina Guanylyl Cyclase (RetGC), RetGC-2
- Creators
- David G. LoweAlexander M. Dizhoor - University of WashingtonKathleen Liu - GenentechQimin GuMaribeth Spencer - University of WashingtonRichard Laura - University of WashingtonLucy LuJames B. Hurley - University of Washington
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 92(12), pp 5535-5539
- Publisher
- National Academy of Sciences of the United States of America
- Number of pages
- 5
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1995RB80400058
- Scopus ID
- 2-s2.0-0029073141
- Other Identifier
- 991022035115304721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology