Journal article
Cloning of a cDNA Encoding MAP1B in Rat Brain: Regulation of mRNA Levels During Development
Journal of neurochemistry, v 52(6), pp 1871-1879
Jun 1989
PMID: 2723641
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Abstract
: This article describes the isolation of a microtubule‐associated protein 1B (MAP1B) cDNA clone from a rat brain λ gt11 library and the study of MAP1B mRNA expression during brain development. On Northern blots, the cDNA hybridized with an mRNA of > 10 kilobases which was present only in the brain. The identity of the cDNA was confirmed by the characterization of the antiserum against the fusion protein, and also by comparing both the original antibody and the anti‐fusion protein antiserum with a panel of well‐studied monoclonal antibodies against different forms of MAP1 and MAP2. The regulation of MAP1B mRNA during development was studied in whole brain, cerebral cortex, hypothalamus, brainstem, and olfactory bulbs. The steady‐state levels of MAP1B mRNA in all tissues examined were relatively low in the adult compared to developing brains. This decrease varied in different brain regions, and its time course appeared to coincide with the pattern of postnatal developmental and morphological events. The developmental patterns of the MAP1B mRNA and protein in the brain were similar, suggesting that expression of this protein is under transcriptional control. The RNA blots were also probed with β‐actin and β‐tubulin to compare the levels of MAP1B mRNA with other cytoskeletal elements and as controls for the quality of the RNA.
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Details
- Title
- Cloning of a cDNA Encoding MAP1B in Rat Brain: Regulation of mRNA Levels During Development
- Creators
- Roohangiz Safaei - Eunice Kennedy Shriver CenterItzhak Fischer - Harvard Medical School
- Publication Details
- Journal of neurochemistry, v 52(6), pp 1871-1879
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 9
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1989U651700028
- Scopus ID
- 2-s2.0-0024348983
- Other Identifier
- 991019203422804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences