Journal article
Control of Muscle Differentiation by a Mitochondria-Targeted Fluorophore
Journal of the American Chemical Society, v 132(2), pp 576-579
20 Jan 2010
PMID: 20020712
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
During muscle differentiation, mitochondria undergo dramatic changes in their morphology and distribution to prepare for the higher rate of energy consumption. By applying a mitochondria-targeted rosamine library in C2C12 myogenesis, we discovered one compound that controls muscle differentiation. When treated to undifferentiated myoblasts, our selected compound, B25, inhibited myotube formation, and when treated to fully differentiated myotubes, it induced fission of multinucleated myotubes into mononucleated fragments. Compared to myoseverin, which is known for inducing myotube fission by destabilizing microtubules, B25 affects neither microtubule stability nor cell cycle. Further investigation identified that B25 induces myotube fission through the activation of NF-kappa B, which is one of the important signaling pathways linked to skeletal muscle differentiation. So far, the use of small-molecule fluorophores is limited in the discovery of labeling agents or sensors. In addition to their potential as a sensor, here we show the application of fluorescent small molecules in the discovery of a bioactive probe that induces a specific cellular response.
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Details
- Title
- Control of Muscle Differentiation by a Mitochondria-Targeted Fluorophore
- Creators
- Yun Kyung Kim - Natl Univ Singapore, Inst Life Sci, Dept Chem, Medchem Program, Singapore 117543, SingaporeHyung-Ho Ha - Natl Univ Singapore, Inst Life Sci, Dept Chem, Medchem Program, Singapore 117543, SingaporeJun-Seok Lee - Natl Univ Singapore, Inst Life Sci, Dept Chem, Medchem Program, Singapore 117543, SingaporeXuezhi Bi - Agency for Science, Technology and ResearchYoung-Hoon Ahn - Natl Univ Singapore, Inst Life Sci, Dept Chem, Medchem Program, Singapore 117543, SingaporeSiti Hajar - Agency for Science, Technology and ResearchJae-Jung Lee - Singapore Bioimaging ConsortiumYoung-Tae Chang - Natl Univ Singapore, Inst Life Sci, Dept Chem, Medchem Program, Singapore 117543, Singapore
- Publication Details
- Journal of the American Chemical Society, v 132(2), pp 576-579
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 4
- Grant note
- R-143-000-353-123 / National University of Singapore (NUS); National University of Singapore KRF-2005-C00088 / Korean government; Korean Government
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Arts and Sciences; Chemistry; Drexel University
- Web of Science ID
- WOS:000275084600033
- Scopus ID
- 2-s2.0-74949122504
- Other Identifier
- 991020100212704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary