Journal article
Decreased Expression of Tissue Inhibitor of Metalloproteinase 1 in Stunned Myocardium
The Journal of surgical research, v 77(1)
01 Jun 1998
PMID: 9698529
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ultrastructural studies of stunned myocardium have shown disorganization and loss of extracellular collagen and increased collagenase activity early after ischemia and reperfusion. The interplay between matrix metalloproteinase 1 (MMP-1) and tissue inhibitor of metalloproteinase 1 (TIMP-1) regulates the turnover of cardiac extracellular matrix fibrillar collagens. However, the gene expression of MMP-1 and TIMP-1 in stunned myocardium is not known. Here, we determined whether altered expression of MMP-1 and TIMP-1 occurs in globally stunned hearts. An isolated nonworking rabbit heart preparation, perfused with a bovine erythrocyte suspension in modified Krebs solution, was used. Two groups were studied: the stunned group was subjected to 20 min of normothermic global ischemia followed by 120 min of normal reperfusion (n= 8), and the control group underwent 140 min of uninterrupted perfusion (n= 7). The developed pressures at the end of reperfusion for ischemic and control hearts were 67.0 ± 2.73 and 83.1 ± 1.52 mm Hg (P< 0.006) respectively. Ribonuclease protection assays of total left ventricular RNA using riboprobes for MMP-1, TIMP-1, and 18S rRNA were performed. A significant decrease (twofold,P< 0.03) in TIMP-1 gene expression was found in the stunned hearts, while MMP-1 mRNA expression was unchanged. Thus, in early stunning, the decrease in TIMP-1 expression could tip the balance favoring enhanced metalloproteinase activity, promoting collagen turnover, and initiating extracellular matrix remodeling. This may contribute to delayed recovery from myocardial stunning.
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Details
- Title
- Decreased Expression of Tissue Inhibitor of Metalloproteinase 1 in Stunned Myocardium
- Creators
- Kourosh Baghelai - Virginia Commonwealth UniversityRalph Marktanner - Virginia Commonwealth UniversityJeffery B. Dattilo - Virginia Commonwealth UniversityMary Peace M. Dattilo - Virginia Commonwealth UniversityEmma R. Jakoi - Virginia Commonwealth UniversityDorne R. Yager - Virginia Commonwealth UniversityRaymond G. Makhoul - Virginia Commonwealth UniversityAndrew S. Wechsler - Virginia Commonwealth University
- Publication Details
- The Journal of surgical research, v 77(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000075267500007
- Scopus ID
- 2-s2.0-0031880103
- Other Identifier
- 991020705450404721
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- Web of Science research areas
- Surgery