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Journal article
Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma
Proteomics (Weinheim), v 16(24), pp 3126-3136
Dec 2016
PMID: 27650323
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in α-1,2, α-1,3, α-1,4, and α-1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of one of the mutated lectins, N224Q lectin. This lectin was characterized by lectin Western blotting, surface plasmon resonance, and glycan microarray and shown to have increased binding to fucosylated glycan. Subsequently, we used this lectin to identify secreted fucosylated glycoproteins from a fetal hepatic cell line. Proteomic analysis revealed several glycoproteins secreted by the fetal cell line that were bound by N224Q lectin. These findings were confirmed by subsequent proteomic analysis of human serum from control patients or patients with hepatocellular carcinoma. These represent candidate oncofetal markers for liver cancer.
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Details
- Title
- Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma
- Creators
- Pamela Norton - Drexel UniversityMary Ann Comunale - Drexel UniversityHarmin Herrera - Drexel UniversityMengjun Wang - Drexel UniversityJosef Houser - Central European Institute of TechnologyMichaela Wimmerova - Central European Institute of TechnologyPatrick R Romano - Baruch S. Blumberg InstituteAnand Mehta - Drexel University
- Publication Details
- Proteomics (Weinheim), v 16(24), pp 3126-3136
- Publisher
- Wiley
- Grant note
- U01 CA168856 / NCI NIH HHS R01 CA120206 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000390809000010
- Scopus ID
- 2-s2.0-85003888949
- Other Identifier
- 991019167973604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology