Journal article
Developmental Regulation of Microtubule‐Associated Protein 2 Expression in Regions of Mouse Brain
Journal of neurochemistry, v 53(6), pp 1910-1917
Dec 1989
PMID: 2809602
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Abstract
: The relative levels of microtubule‐associated protein 2 (MAP2) were determined during postnatal development of the mouse in six different discrete brain regions: cerebellum, cortex, hippocampus, olfactory bulb, brainstem, and hypothalamus. Brain homogenates were electrophoresed on sodium dodecyl sulfate‐containing gels and analyzed by im‐munoblotting with MAP2‐specific antibodies. The levels of MAP2 in each region were determined using radiolabeled secondary antibodies and densitometric quantification of the autoradiograms over a range that was determined to have a linear response. The results indicated that in all regions and at all ages there was only one high‐molecular‐weight polypeptide of MAP2, which did not change in electrophoretic mobility after dephosphorylation. In most regions, the levels of MAP2 increased during the first 2 postnatal weeks. However, there were differences in the time course and relative levels of MAP2 between regions. In addition, all regions of the brain expressed the low‐molecular‐weight form of MAP2 (MAP2c) that was present at birth as a heterogeneous group of polypeptides with an apparent molecular weight of 70K. Most of the heterogeneity of MAP2c, however, was eliminated after dephosphorylation. The levels of MAP2c decreased dramatically after 2 weeks postnatally, except for the olfactory bulb, where the levels of MAP2c remained relatively high even in adults.
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Details
- Title
- Developmental Regulation of Microtubule‐Associated Protein 2 Expression in Regions of Mouse Brain
- Creators
- James E. Crandall - Eunice Kennedy Shriver CenterItzhak Fischer - Harvard University
- Publication Details
- Journal of neurochemistry, v 53(6), pp 1910-1917
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1989AZ79300033
- Scopus ID
- 2-s2.0-0024433649
- Other Identifier
- 991019203335404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences