Journal article
Distribution of Big tau in the central nervous system of the adult and developing rat
Journal of comparative neurology (1911), v 358(2), pp 279-293
24 Jul 1995
PMID: 7560287
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Abstract
The diversity of neuronal morphology and function is correlated with specific expression of various microtubule associated proteins (MAPs). One of the major neuronal MAPs, tau, has multiple isoforms formed as a result of alternative splicing and phosphorylation that are differentially expressed during development. Big tau is a high molecular weight isoform that contains an additional large exon (4a) and is expressed primarily by neurons in the peripheral nervous system (PNS). We cloned the complete 4a exon in an expression vector, isolated the recombinant protein and produced antibodies specific to Big tau that were used to localize Big tau in the developing spinal cord and in the adult central nervous system (CNS). In developing spinal cord, Big tau is first expressed in the central projections of the dorsal root ganglia neurons and in motor neurons at embryonic day 18 and postnatal day 2, respectively. In the adult rat CNS, almost all neurons that extend processes into the PNS express Big tau, including all cranial nerve motor nuclei and central processes of most sensory ganglia; of these ganglia, only the bipolar neurons of the olfactory, vestibular and spiral ganglia did not express Big tau. Retinal ganglion cells are the only CNS neurons, whose processes remain entirely within the CNS, that express high levels of Big tau. The limited and specific distribution of Big tau is consistent with a role in stabilizing microtubules in axons that are subjected to great shear forces.
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Details
- Title
- Distribution of Big tau in the central nervous system of the adult and developing rat
- Creators
- L J Boyne - Drexel University, Medical College of Pennsylvania (1970-1993)A Tessler - Drexel University, Medical College of Pennsylvania (1970-1993)M Murray - Drexel University, Medical College of Pennsylvania (1970-1993)I Fischer - Drexel University, Medical College of Pennsylvania (1970-1993)
- Publication Details
- Journal of comparative neurology (1911), v 358(2), pp 279-293
- Publisher
- Wiley; United States
- Number of pages
- 15
- Grant note
- NS07287 / NINDS NIH HHS NS24707 / NINDS NIH HHS NS24725 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1995RJ97900008
- Scopus ID
- 2-s2.0-0028982504
- Other Identifier
- 991014878299904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences
- Zoology