Journal article
Expression of a Plasma Membrane Proteolipid During Differentiation of Neuronal and Glial Cells in Primary Culture
Journal of neurochemistry, v 47(3), pp 697-706
Sep 1986
PMID: 3016181
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
: Plasma membrane proteolipid protein (PMPLP) synthesis was examined in embryonic rat neurons and neonatal rat glial cells during differentiation in culture. Glial cultures were treated with 1 mM N6,O2, dibutyryl cyclic adenosine monophosphate (dbcAMP) following confluency to induce differentiation, which resulted in the elaboration of long cellular processes. However, no changes in the biosynthetic level of PMPLP was observed during the differentiation of these cells. Neurons differentiated spontaneously in culture, forming cellular aggregates immediately following plating and elaborating a network of neurites over 7 days. The differentiation of neurons was accompanied by a sevenfold increase in PM‐PLP synthesis with increases in biosynthetic rate observed betvyeen days 1 and 3 and between days 3 and 7 in culture. Ultrastructural examination of neurons indicated that the Golgi apparatus was also developing during this period of time, with an increase in both the number of lamellae and generation of vesicles. The transport of PM‐PLP to the plasma membrane was therefore examined in neurons at day 7 in culture by pulse labeling experiments with monensin and colchicine. Monensin (1 μM) was found to inhibit the appearance of radiolabeled PM‐PLP in the plasma membrane by 63%, indicating that a functional Golgi apparatus is required for transport of PM‐PLP to its target membrane. Colchicine (125 μM) also inhibited the appearance of newly synthesized PM‐PLP in the plasma membrane by >40%, suggesting that microtubules may also be required for PM‐PLP transport to the plasma membrane.
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Details
- Title
- Expression of a Plasma Membrane Proteolipid During Differentiation of Neuronal and Glial Cells in Primary Culture
- Creators
- Thomas B. Shea - Department of Biochemistry, E. K. Shriver Center, Waltham, and Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts, U.S.A.Itzhak Fischer - Harvard Medical SchoolVictor Sapirstein - Department of Biochemistry, E. K. Shriver Center, Waltham, and Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts, U.S.A.
- Publication Details
- Journal of neurochemistry, v 47(3), pp 697-706
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1986D635500007
- Scopus ID
- 2-s2.0-0022529279
- Other Identifier
- 991019203772504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences