Journal article
GCAP1, Rab6, and HSP27: Novel Autoantibody Targets in Cancer-Associated Retinopathy and Autoimmune Retinopathy
Translational vision science & technology, v 5(3), 1
01 May 2016
PMID: 27152249
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Purpose: Autoantibodies (AAbs) with different retinal specificities were reported in cancer-associated retinopathy (CAR) and autoimmune retinopathy (AR). The goal was to identify the small retinal proteins of apparent molecular mass of 23-kDa often recognized by patients' AAbs.
Methods: Sera specific for a 23-kDa retinal protein of 173 patients were investigated retrospectively by Western blotting and double immunofluorescence confocal microscopy. A proteomic analysis revealed new 23-kDa protein candidates, including guanylyl cyclase-activating proteins (GCAPs), heat shock protein 27 (HSP27), and Rab6A GTPase (Rab6A).
Results: Among the cohort of 173 patients, only 68 had anti-recoverin AAbs and the remaining 105 reacted with 4 unique proteins, which were identified as a Rab6A, HSP27, GCAP1, and GCAP2. Confocal images from a double labeling study confirmed the reactivity of AAbs with different types of cells in human retina, consistent with the target protein's respective cellular functions. Patients (62/173) had been diagnosed with various kinds of cancer, including 20% of patients who had anti-recoverin, 11% anti-Rab6A, and 5% anti-HSP27 AAbs. Only 50% of recoverin-seropositive patients had cancer and the individuals with anti-recoverin AAbs had a significantly higher likelihood to be diagnosed with cancer than patients with other anti-23-kDa AAbs.
Conclusions: The newly discovered retinal autoantigens may be involved in pathogenicity of CAR and AR. The recognition of AAbs against various retinal proteins associated with autoimmune retinal degeneration broadens the group of proteins related with these entities.
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Details
- Title
- GCAP1, Rab6, and HSP27: Novel Autoantibody Targets in Cancer-Associated Retinopathy and Autoimmune Retinopathy
- Creators
- Sufang Yang - Oregon Health & Science UniversityAlexander Dizhoor - Salus UniversityDavid J. Wilson - Oregon Health & Science UniversityGrazyna Adamus - Oregon Health & Science University
- Publication Details
- Translational vision science & technology, v 5(3), 1
- Publisher
- Assoc Research Vision Ophthalmology Inc
- Number of pages
- 12
- Grant note
- P30EY010572; P30CA069533 / NIH Core Grant; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01EY011522 / NATIONAL EYE INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI) Research to Prevent Blindness (New York, NY, USA); Research to Prevent Blindness (RPB) EY 11522 / National Institutes of Health (NIH; Bethesda, MD, USA); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30CA069533 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000388669900001
- Scopus ID
- 2-s2.0-85042898482
- Other Identifier
- 991022035114804721
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- Web of Science research areas
- Ophthalmology