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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Grafting of Human Bone Marrow Stromal Cells Into Spinal Cord Injury A Comparison of Delivery Methods
Spine (Philadelphia, Pa. 1976), v 34(4), pp 328-334
15 Feb 2009
PMID: 19182705
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Study Design. Three groups of 6 rats received subtotal cervical spinal cord hemisections followed with marrow stromal cell (MSC) transplants by lumbar puncture (LP), intravenous delivery (IV), or direct injection into the injury (control). Animals survived for 4 or 21 days.
Objective. Cell therapy is a promising strategy for the treatment of spinal cord injury (SCI). The mode of cell delivery is crucial for the translation to the clinic. Injections directly into the parenchyma may further damage already compromised tissue; therefore, less invasive methods like LP or IV delivery are preferable.
Summary of Background Data. Human MSC are multipotent mesenchymal adult stem cells that have a potential for autologous transplantation, obviating the need for immune suppression. Although previous studies have established that MSC can be delivered to the injured spinal cord by both LP and IV, the efficacy of cell delivery has not been directly compared with respect to efficacy of delivery and effects on the host.
Methods. Purified MSC from a human donor were transplanted into the CSF at the lumbar region (LP), into the femoral vein (IV), or directly into the injury (control). After sacrifice, spinal cord sections were analyzed for MSC graft size, tissue sparing, host immune response, and glial scar formation, using specific antibodies and Nissl-myelin staining.
Results. LP delivery of MSC to the injured spinal cord is superior to IV delivery. Cell engraftment and tissue sparing were significantly better after LP delivery, and host immune response after LP delivery was reduced compared with IV delivery.
Conclusion. LP is an ideal minimally invasive technique to deliver cellular transplants to the injured spinal cord. It is superior to IV delivery and, together with the potential for autologous transplantation, lends itself for clinical application.
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Details
- Title
- Grafting of Human Bone Marrow Stromal Cells Into Spinal Cord Injury A Comparison of Delivery Methods
- Creators
- Courtney Paul - Drexel UniversityAmer F. Samdani - Shriners Hosp Children, Philadelphia, PA USARandal R. Betz - Shriners Hosp Children, Philadelphia, PA USAItzhak Fischer - Drexel UniversityBirgit Neuhuber - Drexel Univ, Coll Med, Dept Neurobiol & Anat, Philadelphia, PA 19129 USA
- Publication Details
- Spine (Philadelphia, Pa. 1976), v 34(4), pp 328-334
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 7
- Grant note
- 8251; 8570 / The Shriners Hospital for Children R21NS049429 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) NS049429 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 4015 / Craig H. Neilsen Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Surgery
- Web of Science ID
- WOS:000263517800005
- Scopus ID
- 2-s2.0-64749093912
- Other Identifier
- 991019168011904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Clinical Neurology
- Orthopedics