Files and links (1)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
Guiding migration of transplanted glial progenitor cells in the injured spinal cord
Scientific reports, v 6(1), pp 22576-22576
14 Mar 2016
PMID: 26971438
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Transplantation of glial-restricted progenitors (GRPs) is a promising strategy for generating a supportive environment for axon growth in the injured spinal cord. Here we explored the possibility of producing a migratory stream of GRPs via directional cues to create a supportive pathway for axon regeneration. We found that the axon growth inhibitor chondroitin sulfate proteoglycan (CSPG) strongly inhibited the adhesion and migration of GRPs, an effect that could be modulated by the adhesion molecule laminin. Digesting glycosaminoglycan side chains of CSPG with chondroitinase improved GRP migration on stripes of CSPG printed on cover glass, although GRPs were still responsive to the remaining repulsive signals of CSPG. Of all factors tested, the basic fibroblast growth factor (bFGF) had the most significant effect in promoting the migration of cultured GRPs. When GRPs were transplanted into either normal spinal cord of adult rats or the injury site in a dorsal column hemisection model of spinal cord injury, a population of transplanted cells migrated toward the region that was injected with the lentivirus expressing chondroitinase or bFGF. These findings suggest that removing CSPG-mediated inhibition, in combination with guidance by attractive factors, can be a promising strategy to produce a migratory stream of supportive GRPs.
Metrics
Details
- Title
- Guiding migration of transplanted glial progenitor cells in the injured spinal cord
- Creators
- Xiao-bing Yuan - Drexel UniversityYing Jin - Drexel UniversityChristopher Haas - Drexel UniversityLihua Yao - Drexel UniversityKazuo Hayakawa - Drexel UniversityYue Wang - Hussman Institute for AutismChunlei Wang - Hussman Institute for AutismItzhak Fischer - Drexel University
- Publication Details
- Scientific reports, v 6(1), pp 22576-22576
- Publisher
- Nature Publishing Group
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000371870500001
- Scopus ID
- 2-s2.0-84962530986
- Other Identifier
- 991019167715304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences