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Journal article
Harnessing neurovascular interaction to guide axon growth
Scientific reports, v 9(Feb (E-published)), pp 2190-2190
18 Feb 2019
PMID: 30778117
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Regulating the intrinsic interactions between blood vessels and nerve cells has the potential to enhance repair and regeneration of the central nervous system. Here, we evaluate the efficacy of aligned microvessels to induce and control directional axon growth from neural progenitor cells in vitro and host axons in a rat spinal cord injury model. Interstitial fluid flow aligned microvessels generated from co-cultures of cerebral-derived endothelial cells and pericytes in a three-dimensional scaffold. The endothelial barrier function was evaluated by immunostaining for tight junction proteins and quantifying the permeability coefficient (similar to 10(-7) cm/s). Addition of neural progenitor cells to the co-culture resulted in the extension of Tuj-positive axons in the direction of the microvessels. To validate these findings in vivo, scaffolds were transplanted into an acute spinal cord hemisection injury with microvessels aligned with the rostral-caudal direction. At three weeks post-surgery, sagittal sections indicated close alignment between the host axons and the transplanted microvessels. Overall, this work demonstrates the efficacy of exploiting neurovascular interaction to direct axon growth in the injured spinal cord and the potential to use this strategy to facilitate central nervous system regeneration.
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Details
- Title
- Harnessing neurovascular interaction to guide axon growth
- Creators
- Paul P. Partyka - Rowan UniversityYing Jin - Drexel UniversityJulien Bouyer - Drexel UniversityAngelica DaSilva - Rowan UniversityGeorge A. Godsey - Rowan UniversityRobert G. Nagele - Rowan UniversityItzhak Fischer - Drexel UniversityPeter A. Galie - Rowan University
- Publication Details
- Scientific reports, v 9(Feb (E-published)), pp 2190-2190
- Publisher
- Springer Nature
- Number of pages
- 10
- Grant note
- Craig H. Nelson Foundation 17SDG33460432 / American Heart Association Scientist Development Grant; American Heart Association
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000458861700023
- Scopus ID
- 2-s2.0-85061751677
- Other Identifier
- 991019167649204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences