Journal article
Identification and development of fucosylated glycoproteins as biomarkers of primary hepatocellular carcinoma
Journal of proteome research, v 8(2), pp 595-602
Feb 2009
PMID: 19099421
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Changes in N-linked glycosylation are known to occur during the development of cancer. For example, we have previously reported changes in N-linked glycosylation that occur with the development of hepatocellular carcinoma (HCC) and, through the use of glycoproteomics, identified many of those proteins containing altered glycan structures. To advance these studies and further explore the glycoproteome, we performed N-linked glycan analysis from serum samples depleted of the major acute phase proteins, followed by targeted lectin extraction of those proteins containing changes in glycosylation. Using this method, changes in glycosylation, specifically increased amounts of core and outer arm fucosylation were observed in the depleted samples. The identities of those proteins containing core and outer arm fucose were identified in the serum patients with HCC. The usefulness of some of these proteins in the diagnosis of HCC was determined through the analysis of over 300 patient samples using a high-throughput plate based approach. Greatest performance was achieved with fucosylated hemopexin, which had an AUROC of 0.9515 with an optimal sensitivity of 92% and a specificity of 92%.
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Details
- Title
- Identification and development of fucosylated glycoproteins as biomarkers of primary hepatocellular carcinoma
- Creators
- Mary Ann Comunale - Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research of Drexel University, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901Mengjun Wang - Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research of Drexel University, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901Julie Hafner - Immunotope, 3805 Old Easton Road, Doylestown, PA 18901Jonathan Krakover - The Institute for Virus and Hepatitis Research, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901Lucy Rodemich - Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research of Drexel University, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901Brent Kopenhaver - Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research of Drexel University, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901Ronald E Long - Immunotope, 3805 Old Easton Road, Doylestown, PA 18901Omer Junaidi - Saint Louis University School of Medicine, 1402 S. Grand FDT 12th Floor, St. Louis, Mo 63104Adrian M Di Bisceglie - Saint Louis University School of Medicine, 1402 S. Grand FDT 12th Floor, St. Louis, Mo 63104Timothy M Block - Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research of Drexel University, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901Anand S Mehta - Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research of Drexel University, Pennsylvania Commonwealth Institute, Doylestown, PA, 18901
- Publication Details
- Journal of proteome research, v 8(2), pp 595-602
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000263193300019
- Scopus ID
- 2-s2.0-61849124770
- Other Identifier
- 991014878069804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods