Journal article
In Vitro Differentiation of Human Marrow Stromal Cells into Early Progenitors of Neural Cells by Conditions That Increase Intracellular Cyclic AMP
Biochemical and biophysical research communications, v 282(1), pp 148-152
23 Mar 2001
PMID: 11263984
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human marrow stromal cells (hMSCs) are multipotential stem cells that can be differentiated into bone, cartilage, fat, and muscle. In the experiments here, we found that undifferentiated cultures of hMSCs express some markers characteristic of neural cells such as microtubule-associated protein 1B (MAP1B), neuron-specific tubulin (TuJ-1), neuron-specific enolase (NSE), and vimentin. By treating hMSCs with 0.5 mM isobutylmethylxanthine (IBMX)/1 mM dibutyryl cyclic AMP (dbcAMP) for 6 days, about 25% of the hMSCs differentiated into cells with a typical neural cell morphology and with increased levels of both NSE and vimentin. The data suggested that the hMSCs may have been differentiated into early progenitors of neural cells in vitro under conditions that increase the intracellular level of cAMP.
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Details
- Title
- In Vitro Differentiation of Human Marrow Stromal Cells into Early Progenitors of Neural Cells by Conditions That Increase Intracellular Cyclic AMP
- Creators
- Weiwen Deng - Center for Gene Therapy, Tulane University Health Sciences Center, SL-99, 1430 Tulane Avenue, New Orleans, Louisiana, 70112Maria Obrocka - Department of Neurobiology and Anatomy, MCP Hahnemann University, 2900 Queen Lane, Philadelphia, Pennsylvania, 19129Itzhak Fischer - Department of Neurobiology and Anatomy, MCP Hahnemann University, 2900 Queen Lane, Philadelphia, Pennsylvania, 19129Darwin J Prockop - Center for Gene Therapy, Tulane University Health Sciences Center, SL-99, 1430 Tulane Avenue, New Orleans, Louisiana, 70112
- Publication Details
- Biochemical and biophysical research communications, v 282(1), pp 148-152
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000167798400024
- Scopus ID
- 2-s2.0-0034809239
- Other Identifier
- 991014878500104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics