Journal article
Influence of alginate cross-linking method on neurite response to microencapsulated neurotrophin-producing fibroblasts
Journal of microencapsulation, v 28(5), pp 353-362
01 Jan 2011
PMID: 21736520
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Transplantation of cells genetically modified to produce neurotrophins is a promising spinal cord repair strategy. Previously we showed that fibroblasts engineered to produce brain-derived neurotrophic factor (Fb/BDNF) microencapsulated in alginate survive, continue to grow and express bioactive BDNF. We here compared the effects of different alginate crosslinkers on dorsal root ganglia (DRG) neurite growth using alginate-encapsulated Fb/BDNF. Aqueous sodium alginate (+/-Fb/BDNF) was contacted with different calcium salts, and used as substrate for DRG growth. Length, number and orientation of neurites were measured. Chloride or carbonate salts promoted significantly more neurite growth than sulphate, with or without Fb/BDNF, although encapsulated Fb/BDNF stimulated significantly more neurite growth than cell-free. An Fb/BDNF-derived neurotrophin concentration gradient directionally guided DRG neurite growth. This positive effect of alginate-encapsulated Fb/BDNF on neurite growth/guidance shows promise for enhanced regeneration and guidance of axons towards a specific target in the injured spinal cord.
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Details
- Title
- Influence of alginate cross-linking method on neurite response to microencapsulated neurotrophin-producing fibroblasts
- Creators
- Nicola L. Francis - Drexel UniversityMihir S. Shanbhag - Drexel UniversityItzhak Fischer - Drexel UniversityMargaret A. Wheatley - Drexel University
- Publication Details
- Journal of microencapsulation, v 28(5), pp 353-362
- Publisher
- Taylor & Francis
- Number of pages
- 10
- Grant note
- Bryon Riesch Paralysis Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000292564400003
- Scopus ID
- 2-s2.0-79960226327
- Other Identifier
- 991019169373104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Applied
- Engineering, Chemical
- Pharmacology & Pharmacy