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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Interaction of retinal guanylate cyclase with the alpha subunit of transducin: potential role in transducin localization
Biochemical journal, v 417(3), pp 803-812
01 Feb 2009
PMID: 18840097
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Vertebrate phototransduction is mediated by cGMP, which is generated by retGC (retinal guanylate cyclase) and degraded by cGMP phosphodiesterase. Light stimulates cGMP hydrolysis via the G-protein transducin, which directly binds to and activates phosphodiesterase. Bright light also causes relocalization of transducin from the OS (outer segments) of the rod cells to the inner compartments. In the present study, we show experimental evidence for a previously unknown interaction between G(alphat) (the transducin alpha subunit) and retGC. G(alphat) co-immunoprecipitates with retGC from the retina or from co-transfected COS-7 cells. The retGC-G(alphat) complex is also present in cones. The interaction also occurs in mice lacking RGS9 (regulator of G-protein signalling 9), a protein previously shown to associate with both G(alphat) and retGC. The G(alphat)-retGC interaction is mediated primarily by the kinase homology domain of retGC, which binds GDP-bound G(alphat) stronger than the GTP[S] (GTPgammaS; guanosine 5'-[gamma-thio]triphosphate) form. Neither G(alphat) nor G(betagamma) affect retGC-mediated cGMP synthesis, regardless of the presence of GCAP (guanylate cyclase activating protein) and Ca2+. The rate of light-dependent transducin redistribution from the OS to the inner segments is markedly accelerated in the retGC-1-knockout mice, while the migration of transducin to the OS after the onset of darkness is delayed. Supplementation of permeabilized photoreceptors with cGMP does not affect transducin translocation. Taken together, these results suggest that the protein-protein interaction between G(alphat) and retGC represents a novel mechanism regulating light-dependent translocation of transducin in rod photoreceptors.
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Details
- Title
- Interaction of retinal guanylate cyclase with the alpha subunit of transducin: potential role in transducin localization
- Creators
- Derek H Rosenzweig - University of MiamiK Saidas Nair - University of MiamiKonstantin Levay - University of MiamiIgor V Peshenko - Salus UniversityJohn W Crabb - Cleveland Eye ClinicAlexander M Dizhoor - Salus UniversityVladlen Z Slepak - University of Miami
- Publication Details
- Biochemical journal, v 417(3), pp 803-812
- Grant note
- R01 GM060019-03 / NIGMS NIH HHS R01 EY012982-04 / NEI NIH HHS R01 GM060019-03S1 / NIGMS NIH HHS R01 EY012982 / NEI NIH HHS R01 GM060019-04 / NIGMS NIH HHS R01 EY012982-01 / NEI NIH HHS R01 GM060019-02S1 / NIGMS NIH HHS R01 GM060019-01 / NIGMS NIH HHS R01 GM060019-05 / NIGMS NIH HHS P30 EY014801 / NEI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Pennsylvania College of Optometry (PCO)
- Web of Science ID
- WOS:000263257700018
- Scopus ID
- 2-s2.0-59849090753
- Other Identifier
- 991022035257404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology