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Isolation of a glial-restricted tripotential cell line from embryonic spinal cord cultures
Journal article   Peer reviewed

Isolation of a glial-restricted tripotential cell line from embryonic spinal cord cultures

Yuan Yuan Wu, Tahmina Mujtaba, Steve S W Han, Itzhak Fischer and Mahendra S Rao
Glia, v 38(1)
01 Apr 2002
DOI: https://doi.org/10.1002/glia.10049
PMID: 11921204
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Oligodendroglia - metabolism Neurons - cytology Stem Cells - cytology Stem Cells - metabolism Cell Culture Techniques - methods Neuroglia - cytology Female Fetus Neurons - metabolism Oligodendroglia - cytology Spinal Cord - cytology Cell Differentiation - physiology Astrocytes - cytology Rats Rats, Sprague-Dawley Genetic Vectors - genetics Spinal Cord - surgery Cell Lineage - physiology Nerve Tissue Proteins - metabolism Pregnancy Spinal Cord - embryology Animals Graft Survival - genetics Genes, myc - genetics Growth Substances - pharmacology Neuroglia - metabolism Brain Tissue Transplantation - methods Cell Line, Transformed Astrocytes - metabolism
Neuroepithelial stem cells (NEPs), glial-restricted precursors (GRPs), and neuron-restricted precursors (NRPs) are present during early differentiation of the spinal cord and can be identified by cell surface markers. In this article, we describe the properties of GRP cells that have been immortalized using a regulatable v-myc retrovirus construct. Immortalized GRP cells can be maintained in an undifferentiated dividing state for long periods and can be induced to differentiate into two types of astrocytes and into oligodendrocytes in culture. A clonal cell line prepared from immortalized GRP cells, termed GRIP-1, was also shown to retain the properties of a glial-restricted tripotential precursor. Transplantation of green fluorescent protein (GFP)-labeled subclones of the immortalized cells into the adult CNS demonstrates that this cell line can also participate in the in vivo development of astrocytes and oligodendrocytes. Late passages of the immortalized cells undergo limited transdifferentiation into neurons as assessed by expression of multiple neuronal markers. The availability of a conditionally immortalized cell line obviates the difficulties of obtaining a large and homogeneous population of GRPs that can be used for studying the mechanism and signals for glial cell differentiation as well as their application in transplantation protocols.

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Collaboration types
Domestic collaboration
Web of Science research areas
Neurosciences
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