Multi‐rate nonlinear state and parameter estimation in a bioreactor

Srinivas Tatiraju; Masoud Soroush; Raj Mutharasan

doi:10.1002/(SICI)1097-0290(19990405)63:1<22::AID-BIT3>3.0.CO;2-2

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Multi‐rate nonlinear state and parameter estimation in a bioreactor

Journal article

Peer reviewed

Multi‐rate nonlinear state and parameter estimation in a bioreactor

Srinivas Tatiraju, Masoud Soroush and Raj Mutharasan

Biotechnology and bioengineering, v 63(1), pp 22-32

05 Apr 1999

DOI: https://doi.org/10.1002/(SICI)1097-0290(19990405)63:1<22::AID-BIT3>3.0.CO;2-2

PMID: 10099578

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Abstract

state estimation

parameter estimation

biochemical reactor

process monitoring

multi‐rate measurements

real‐time implementation

This paper concerns real‐time, multi‐rate, nonlinear state and parameter estimation in a pilot‐scale biochemical reactor in which cultivation of mouse–mouse hybridoma cells takes place. A multi‐rate estimator is designed and implemented to estimate specific growth rate and concentrations of viable cells, total cells, glucose, glutamine, and monoclonal antibodies (MAb) in the reactor. These are estimated from frequent measurement (inferred values) of oxygen uptake rate (OUR) and infrequent and delayed measurements of the concentrations of viable cells, total cells, glucose, glutamine, and MAb. The infrequent measurements are available every 2 to 17 h with a time delay of 0.08 to 2.00 h, and OUR is inferred from dissolved oxygen concentration measurements that are available very 0.17 h. For each of the process variables, its infrequent measurement data and the profile of its estimate are presented to show the performance of the multi‐rate estimator. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 22–32, 1999.