Journal article
N-Linked Glycosylation of the Liver Cancer Biomarker GP73
Journal of cellular biochemistry, v 104(1)
01 May 2008
PMID: 18004786
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The association between elevated circulating levels of GP73 (and fucosylated GP73 in particular) and hepatocellular carcinoma suggests that a thorough analysis of the extent of GP73 glycosylation is warranted. Detailed analysis of the glycosylation patterns of such low abundance proteins are hampered by technical difficulties. Using conventional lectin affinity chromatography, we have established that three quarters of the GP73 secreted from a cell line derived from HCC is fucosylated. Using mass spectrometry, we have established that at least two of three potential sites of N-linked glycosylation are occupied on most molecules of GP73 secreted from cultured hepatoma cells. Furthermore, the oligosaccharides added to recombinant GP73 resemble those present in the bulk of secreted protein, mostly bi-antennary with core fucose, with a smaller fraction of tri- and tetra-antennary structures. The frequency of fucosylation observed on the recombinant protein agrees well with the pattern of lectin binding of the endogenous secreted protein. Finally, we have developed a method to interrogate the glycans added to either the near full length protein or at a particular sequon, providing proof of concept that a small peptide embedded in a heterologous context can preserve both fucosylation and a high level of branching of oligosaccharides added.
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Details
- Title
- N-Linked Glycosylation of the Liver Cancer Biomarker GP73
- Creators
- Pamela A Norton - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaMary Ann Comunale - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaJonathan Krakover - Institute for Hepatitis and Virus Research, Doylestown, PennsylvaniaLucy Rodemich - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaNatalie Pirog - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaAnthony D'Amelio - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaRamila Philip - Institute for Hepatitis and Virus Research, Doylestown, PennsylvaniaAnand S Mehta - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaTimothy M Block - Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania
- Publication Details
- Journal of cellular biochemistry, v 104(1)
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000260277000013
- Scopus ID
- 2-s2.0-46449102461
- Other Identifier
- 991014877679204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology