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Permeability and morphology of low temperature phases in bilayers of single and of mixtures of phosphatidylcholines
Journal article   Peer reviewed

Permeability and morphology of low temperature phases in bilayers of single and of mixtures of phosphatidylcholines

Michael A. Singer and Leonard Finegold
Biochimica et biophysica acta. Biomembranes, v 816(2), pp 303-312
1985
PMID: 4005246

Abstract

Differential scanning calorimetry Freeze-fracture Membrane permeability Phase transition Phospholipid bilayer Surface morphology
The properties of subtransitions were studied in aqueous dispersions of saturated phosphatidylcholines (PC) by means of permeability measurements, freeze-fracture electron microscopy, and differential scanning calorimetry (DSC). For dispersions of C 16PC, a C 16PC analog (2,3-dipalmitoyl-cyclopentano-1-phosphocholine with four methylene residues between the nitrogen and the phosphorus atoms) and C 17PC, there was good agreement between phase properties (including subtransitions) as observed by DSC and temperature-related permeability. C 16PC and C 17PC dispersions also displayed a ‘crinkled’ surface morphology in the subgel state. The phase diagram for mixtures of C 14PC and C 16PC was consistent with ideal mixing of these two components in the subgel state and also illustrated the relative independence of the subtransition on acyl chain length as compared to the pre- and main transitions. Together, these results indicate that (i) permeability, DSC and freeze-fracture electron microscopy measurements do correlate reasonably well with the existence of a subgel state, (ii) mixtures of lipids with similar acyl chain lengths can be used to investigate subtransitions, (iii) the development of a subtransition appears to be mainly a function of the non-acyl chain moiety of the phospholipid.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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