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Proteomic analysis of serum associated fucosylated glycoproteins in the development of primary hepatocellular carcinoma
Journal article   Peer reviewed

Proteomic analysis of serum associated fucosylated glycoproteins in the development of primary hepatocellular carcinoma

Mary Ann Comunale, Melissa Lowman, Ronald E Long, Jonathan Krakover, Ramila Philip, Steven Seeholzer, Alison A Evans, Hie-Won L Hann, Timothy M Block and Anand S Mehta
Journal of proteome research, v 5(2), pp 308-315
Feb 2006
DOI: https://doi.org/10.1021/pr050328x
PMID: 16457596
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Liver Neoplasms - virology Serum - metabolism Humans Middle Aged Liver Neoplasms - complications Electrophoresis, Gel, Two-Dimensional Male Carcinoma, Hepatocellular - virology Glycoproteins - blood Glycosylation Hepatitis B - complications Carcinoma, Hepatocellular - complications Immunoglobulins - blood Liver Neoplasms - metabolism Biomarkers, Tumor - metabolism Female Carcinoma, Hepatocellular - metabolism
Changes in N-linked glycosylation are known to occur during the development of cancer. For example, increased branching of oligosaccharides has been associated with metastasis and has been correlated to tumor progression in human cancers of the breast, colon and melanomas. Increases in core fucosylation have also been associated with the development of hepatocellular carcinoma (HCC). Chronic infection with the hepatitis B virus is associated with more than 55% of all cases of hepatocellular carcinoma. We show here that increased levels of core fucosylation can be observed via glycan analysis of total serum and are associated with the development of HCC. In a blinded study, the serum glycoproteins derived from people diagnosed with HBV induced liver cancer were found to possess a dramatically higher level of fucosylation. This change occurs on both immunoglobulin molecules and on other serum glycoproteins. Targeted glycoproteomic analysis was used to identify those glycoproteins that are hyperfucosylated in cancer. In total, 19 proteins were found to be hyperfucosylated in cancer. The potential of these proteins as biomarkers of cancer is discussed.

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189 citations in Web of Science
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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemical Research Methods
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