Journal article
RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function
Chemistry & biology, v 13(6), pp 615-623
2006
PMID: 16793519
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The higher-order structural organization of the cell nucleus reflects the underlying genome-wide transcriptional activity and macromolecular transport processes. To study the microscopic organization of RNA distribution within the nucleus, a combinatorial library of fluorescent styryl molecules was synthesized and screened for an in vitro RNA response and live cell nuclear imaging. Four different cell lines (HeLa, A549, 3T3, and 3T3-L1) were analyzed in terms of higher-order nuclear organization. We identified RNA-selective dyes with better imaging properties relative to commercially available SYTORNASelect dye; the selected dyes were also cell permeant, photostable, and well tolerated by the cells. Our dyes also had very good counterstain compatibility with Hoechst and DAPI, which could help to image the DNA distribution in relation to RNA distribution in live cells and therefore reveal different patterns of RNA-DNA colocalization.
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Details
- Title
- RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function
- Creators
- Qian Li - New York UniversityYunkyung Kim - Department of Chemistry, New York University, New York, New York 10003Joshua Namm - New York UniversityAmita Kulkarni - New York UniversityGus R. Rosania - University of MichiganYoung-Hoon Ahn - Department of Chemistry, New York University, New York, New York 10003Young-Tae Chang - Department of Chemistry, New York University, New York, New York 10003
- Publication Details
- Chemistry & biology, v 13(6), pp 615-623
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Arts and Sciences; Chemistry; Drexel University
- Web of Science ID
- WOS:000238723800010
- Scopus ID
- 2-s2.0-33745202571
- Other Identifier
- 991020099238404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology