Journal article
Radial Glial Cell Line C6-R Integrates Preferentially in Adult White Matter and Facilitates Migration of Coimplanted Neurons in Vivo
Experimental neurology, v 168(2), pp 310-322
Apr 2001
PMID: 11259119
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
C6-R is a cell line derived from C6 glioma cells that exhibits key properties of radial glia including the ability to support neuronal migration in culture. To explore its potential use in promoting neuronal migration in vivo, we analyzed the behavior of C6-R cells in the intact and injured adult rat CNS. At 6–11 days postimplantation at the splenium of the corpus callosum, green fluorescent protein-labeled C6-R cells were observed primarily in either the corpus callosum or the hippocampus in the brain, and in the spinal cord they migrated more extensively in the white matter than in the grey matter. To determine whether C6-R cells retain their ability to promote neuronal migration in vivo, they were coinjected with labeled neurons into adult brain. When rat embryonic neurons were coimplanted with C6-R cells, the neurons and C6-R cells comigrated through a much larger volume than neurons alone or neurons coimplanted with fibroblasts. In brains preinjured with ibotenic acid, C6-R cells as well as coimplanted neurons distributed widely within the lesion site and migrated into adjacent brain tissue, while transplants with neurons alone were restricted primarily to the lesion site. The results suggest that radial glial cell lines can serve as a scaffold for neuronal migration that may facilitate development of experimental models for neural transplantation and regeneration.
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Details
- Title
- Radial Glial Cell Line C6-R Integrates Preferentially in Adult White Matter and Facilitates Migration of Coimplanted Neurons in Vivo
- Creators
- Adı́lia Hormigo - New York UniversityMaria McCarthy - New York UniversityJean Manuel Nothias - Drexel UniversityKoichi Hasegawa - Rutgers, The State University of New JerseyWencheng Huang - Rutgers, The State University of New JerseyDavid R. Friedlander - New York UniversityItzhak Fischer - Drexel UniversityGord Fishell - New York University Langone Medical CenterMartin Grumet - Department of Pharmacology, NYU Medical Center, 550 First Avenue, New York, New York, 10016Matthew McCarthy - Mechanical Engineering and Mechanics
- Publication Details
- Experimental neurology, v 168(2), pp 310-322
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000167904100012
- Scopus ID
- 2-s2.0-0035076032
- Other Identifier
- 991019169569004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences