Journal article
Recovery of Function Following Grafting of Human Bone Marrow-Derived Stromal Cells into the Injured Spinal Cord
Neurorehabilitation and neural repair, v 20(2), pp 278-296
Jun 2006
PMID: 16679505
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study evaluates functional recovery after transplanting human bone marrow-derived stromal cells (BMSCs) into contusion models of spinal cord injury (SCI). The authors used a high-throughput process to expand BMSCs and characterized them by flow cytometry, ELISA, and gene expression. They found that BMSCs secrete neurotrophic factors and cytokines with therapeutic potential for cell survival and axon growth. In adult immune-suppressed rats, mild, moderate, or severe contusions were generated using the MASCIS impactor. One week following injury, 0.5 to 1 × 106 BMSCs were injected into the lesioned spinal cord; control animals received vehicle injection. Biweekly behavioral tests included the Basso, Beattie, and Bresnahan Locomotor Rating Scale (BBB), exploratory rearing, grid walking, and thermal sensitivity. Animals receiving moderate contusions followed by BMSC grafts showed significant behavioral recovery in BBB and rearing tests when compared to controls. Animals receiving BMSC grafts after mild or severe contusion showed trends toward improved recovery. Immunocytochemistry identified numerous axons passing through the injury in animals with BMSC grafts but few in controls. BMSCS were detected at 2 weeks after transplantation; however, at 11 weeks very few grafted cells remained. The authors conclude that BMSCs show potential for repairing SCI. However, the use of carefully characterized BMSCs improved transplantation protocols ensuring BMSC, survival, and systematic motor and sensory behavioral testing to identify robust recovery is imperative for further improvement.
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Details
- Title
- Recovery of Function Following Grafting of Human Bone Marrow-Derived Stromal Cells into the Injured Spinal Cord
- Creators
- B. Timothy Himes - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, Department of Veterans Affairs Hospital, Philadelphia, PABirgit Neuhuber - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PACarl Coleman - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PARobert Kushner - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PASharon A Swanger - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PAGene C Kopen - Neuronyx Inc., Malvern, PAJoseph Wagner - Neuronyx Inc., Malvern, PAJed S Shumsky - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PAItzhak Fischer - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA
- Publication Details
- Neurorehabilitation and neural repair, v 20(2), pp 278-296
- Publisher
- Sage Publications; Thousand Oaks, CA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000237627300004
- Scopus ID
- 2-s2.0-33646515566
- Other Identifier
- 991014878360704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Clinical Neurology
- Rehabilitation