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Regulation of microtubule associated protein 2 (MAP2) expression by nerve growth factor in PC12 cells
Journal article   Peer reviewed

Regulation of microtubule associated protein 2 (MAP2) expression by nerve growth factor in PC12 cells

Itzhak Fischer, Christiane Richter-Landsberg and Roohangiz Safaei
Experimental cell research, v 194(2), pp 195-201
1991
DOI: https://doi.org/10.1016/0014-4827(91)90354-W
PMID: 2026175
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

In the presence of nerve growth factor (NGF), PC12 cells cease to divide and differentiate, extending long microtubule-containing neurites. We showed by immunoblot analysis that MAP2 was detectable in PC12 after 4 days of NGF treatment and that its levels increased five- to sevenfold after 12 days of NGF treatment. The apparent molecular weight of MAP2 in PC12 cells was similar to that of rat brain MAP2 (280,000), with a doublet representing the MAP2 isoforms. However, the relative levels of MAP2 in differentiated PC12 cells were 5–10% of those found in rat brain. Immunofluorescence analysis of NGF-treated PC12 cells revealed that MAP2 co-localized with tubulin and was present in cell bodies and neurites. Northern blot analysis showed that the levels of MAP2 mRNA increased in PC12 cells during NGF-treatment in a pattern that paralleled the protein levels, suggesting that MAP2 expression is transcriptionally regulated.

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Cell Biology
Oncology
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