Journal article
Retinal degeneration-3 protein attenuates photoreceptor degeneration in transgenic mice expressing dominant mutation of human retinal guanylyl cyclase
The Journal of biological chemistry, v 297(4), pp 101201-101201
01 Oct 2021
PMID: 34537244
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Different forms of photoreceptor degeneration cause blindness. Retinal degeneration-3 protein (RD3) deficiency in photoreceptors leads to recessive congenital blindness. We proposed that aberrant activation of the retinal membrane guanylyl cyclase (RetGC) by its calcium-sensor proteins (guanylyl cyclase-activating protein [GCAP]) causes this retinal degeneration and that RD3 protects photoreceptors by preventing such activation. We here present in vivo evidence that RD3 protects photoreceptors by suppressing activation of both RetGC1 and RetGC2 isozymes. We further suggested that insufficient inhibition of RetGC by RD3 could contribute to some dominant forms of retinal degeneration. The R838S substitution in RetGC1 that causes autosomal-dominant cone- rod dystrophy 6, not only impedes deceleration of RetGC1 activity by Ca(2+)GCAPs but also elevates this isozyme's resistance to inhibition by RD3. We found that RD3 prolongs the survival of photoreceptors in transgenic mice harboring human R838S RetGC1 (R838S(+)). Overexpression of GFP-tagged human RD3 did not improve the calcium sensitivity of cGMP production in R838S(+) retinas but slowed the progression of retinal blindness and photoreceptor degeneration. Fluorescence of the GFP-tagged RD3 in the retina only partially overlapped with immunofluorescence of RetGC1 or GCAP1, indicating that RD3 separates from the enzyme before the RetGC1:GCAP1 complex is formed in the photoreceptor outer segment. Most importantly, our in vivo results indicate that, in addition to the abnormal Ca2+ sensitivity of R838S RetGC1 in the outer segment, the mutated RetGC1 becomes resistant to inhibition by RD3 in a different cellular compartment(s) and suggest that RD3 overexpression could be utilized to reduce the severity of cone-rod dystrophy 6 pathology.
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Details
- Title
- Retinal degeneration-3 protein attenuates photoreceptor degeneration in transgenic mice expressing dominant mutation of human retinal guanylyl cyclase
- Creators
- Igor V. Peshenko - Salus UniversityElena V. Olshevskaya - Salus UniversityAlexander M. Dizhoor - Salus University
- Publication Details
- The Journal of biological chemistry, v 297(4), pp 101201-101201
- Publisher
- Elsevier
- Number of pages
- 15
- Grant note
- CURE Formula grant from Pennsylvania Department of Health EY11522 / National Institutes of Health from National Eye Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Pennsylvania College of Optometry (PCO)
- Web of Science ID
- WOS:000713003600005
- Scopus ID
- 2-s2.0-85116717517
- Other Identifier
- 991022035116104721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology