Journal article
Role of vimentin in early stages of neuritogenesis in cultured hippocampal neurons
International journal of developmental neuroscience, v 14(6), pp 739-748
Dec 1996
PMID: 8960981
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Abstract
Vimentin is expressed initially by nearly all neuronal precursors in vivo, and is replaced by neurofilaments shortly after the immature neurons become post‐mitotic. Moreover, both vimentin and neurofilaments can be detected transiently within the same neurite, leaving open the possibility that vimentin may play a role in the early stages of neuritogenesis. In the present study, cultured hippocampal neurons, which transiently express vimentin in culture, were treated with sense‐ and antisense‐oriented deoxyoligonucleotides encoding regions of the vimentin sequence that overlap the translation initiation codon. Antisense oligonucleotide treatment reduced vimentin‐immunoreactivity to background levels. Moreover, while 90–100% of cultured hippocampal neurons elaborated neurites within the first 24 hr following plating, only 24–30% did so in the presence of vimentin antisense oligonucleotides. Inhibition of neurite outgrowth was reversible following removal of antisense oligonucleotide. These findings substantiate earlier studies in neuroblastoma cells, indicating a possible role for vimentin in the initiation of neurite outgrowth.
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Details
- Title
- Role of vimentin in early stages of neuritogenesis in cultured hippocampal neurons
- Creators
- Lesley J Boyne - Medical College of PennsylvaniaItzhak Fischer - Medical College of PennsylvaniaThomas B Shea - University of Massachusetts at Lowell
- Publication Details
- International journal of developmental neuroscience, v 14(6), pp 739-748
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- NSF (BNS8910869; AG05134; MH47349; NS27425)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1996VX89700006
- Scopus ID
- 2-s2.0-0030569605
- Other Identifier
- 991014878547304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Developmental Biology
- Neurosciences