Journal article
Small-molecule fluorophores to detect cell-state switching in the context of high-throughput screening
Journal of the American Chemical Society, v 130(13), pp 4208-4209
02 Apr 2008
PMID: 18327938
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A small molecule capable of distinguishing the distinct states resulting from cellular differentiation would be of enormous value, for example, in efforts aimed at regenerative medicine. We screened a collection of fluorescent small molecules for the ability to distinguish the differentiated state of a mouse skeletal muscle cell line. High-throughput fluorescence-based screening of C2C12 myoblasts and myotubes resulted in the identification of six compounds with the desired selectivity, which was confirmed by high-content screening in the same cell states. The compound that resulted in the greatest fluorescence intensity difference between the cell states was used as the screening agent in a pilot screen of 84 kinase inhibitors, each present in four doses, for inhibition of myogenesis. Of the kinase inhibitors, 17 resulted in reduction of fluorescence at one or more concentrations; among the "hits" included known inhibitors of myogenesis, confirming that this compound is capable of detecting the differentiated myotube state. We suggest that the strategy of screening for screening agents reported here may be extended more broadly in the future.
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Details
- Title
- Small-molecule fluorophores to detect cell-state switching in the context of high-throughput screening
- Creators
- Bridget K. Wagner - Agency for Science, Technology and ResearchHyman A. Carrinski - Agency for Science, Technology and ResearchYoung-Hoon Ahn - Agency for Science, Technology and ResearchYun Kyung Kim - NYU, Dept Chem, New York, NY 10003 USATamara J. Gilbert - Agency for Science, Technology and ResearchDina A. Fomina - Agency for Science, Technology and ResearchStuart L. Schreiber - Agency for Science, Technology and ResearchYoung-Tae Chang - Agency for Science, Technology and ResearchPaul A. Clemons - Agency for Science, Technology and Research
- Publication Details
- Journal of the American Chemical Society, v 130(13), pp 4208-4209
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 3
- Grant note
- DK060837 / NIDDK NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R01DK060837 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) P20-GM072029 / NIGMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) P20GM072029 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) Howard Hughes Medical Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Arts and Sciences; Chemistry; Drexel University
- Web of Science ID
- WOS:000254549000001
- Scopus ID
- 2-s2.0-41549155107
- Other Identifier
- 991020099925404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary