Journal article
Stem cell delivery by lumbar puncture as a therapeutic alternative to direct injection into injured spinal cord
Journal of neurosurgery. Spine, v 9(4), pp 390-399
Oct 2008
PMID: 18939929
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Using cellular transplants to treat spinal cord injury is a promising therapeutic strategy, but transplants grafted directly into the injury site can further damage the already compromised cord. To avoid additional trauma and to simplify translation to the clinic, it is advantageous to use less invasive delivery methods.
The authors compared the efficacy of intrathecal cell delivery at the lumbar region (lumbar puncture [LP]) to direct injection into a thoracic contusion injury using a mixed population of lineage-restricted neural precursor cells.
Direct injection resulted in a higher volume of neural precursor cells located throughout the injury site, whereas fewer LP-delivered cells accumulated at the dorsal aspect of the injured cord. Both grafting methods were neuroprotective, resulting in reduction of injury size and greater tissue sparing compared with controls. Functional recovery was evaluated by assessing motor and bladder function. Animals that received cells via direct injection performed significantly better in the open-field locomotor test than did operated controls, while LP-treated animals showed intermediate recovery of function that did not differ statistically from that of either operated controls or directly injected animals. Bladder function, however, was significantly improved in both directly injected and LP-treated animals.
Grafting of stem cells via LP resulted in localized accumulation of cells at the injury site, neuroprotection, and modest recovery of function. Further optimization of the LP procedure by increasing the number of cells that are delivered and determining the optimal delivery schedule may further improve recovery to levels comparable to direct injection.
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Details
- Title
- Stem cell delivery by lumbar puncture as a therapeutic alternative to direct injection into injured spinal cord
- Creators
- Birgit Neuhuber - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA. bneuhuber@drexelmed.eduAlissa L BarshingerCourtney PaulJed S ShumskyTakahiko MitsuiItzhak Fischer
- Publication Details
- Journal of neurosurgery. Spine, v 9(4), pp 390-399
- Publisher
- United States
- Grant note
- NS049429 / NINDS NIH HHS NS24707 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000259549400014
- Scopus ID
- 2-s2.0-85047686295
- Other Identifier
- 991014878214804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Clinical Neurology
- Surgery