Journal article
Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein
Nucleic acids research, v 42(9), pp 6025-6037
14 May 2014
PMID: 24688060
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses.
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Details
- Title
- Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein
- Creators
- Daniel Olal - Max Delbrück CenterAlexej Dick - Max Delbrück CenterVirgil L. Woods - University of California San DiegoTong Liu - University of California San DiegoSheng Li - University of California San DiegoStephanie Devignot - Philipps University of MarburgFriedemann Weber - Philipps University of MarburgErica Ollmann Saphire - Scripps Research InstituteOliver Daumke - Max Delbrück Center
- Publication Details
- Nucleic acids research, v 42(9), pp 6025-6037
- Publisher
- Oxford University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000336495400059
- Scopus ID
- 2-s2.0-84901347557
- Other Identifier
- 991021229991904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology