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The formation of multilamellar vesicles from saturated phosphatidylcholines and phosphatidylethanolamines: morphology and quasi-elastic light scattering measurements
Journal article   Peer reviewed

The formation of multilamellar vesicles from saturated phosphatidylcholines and phosphatidylethanolamines: morphology and quasi-elastic light scattering measurements

Michael A. Singer, Leonard Finegold, Paul Rochon and Thomas J. Racey
Chemistry and physics of lipids, v 54(2), pp 131-146
1990
PMID: 2364473

Abstract

light scattering phospholipid vesicle formation
The aggregation properties of diacyl phosphatidylcholines (PC) and phosphatidylethanolamines (PE), with linear symmetrical saturated chains, were characterized at temperatures below and above the lipid solid to fluid transition. PEs in the solid state form bundles of closely apposed flat bilayer stacks which at the solid to fluid transition temperature fold into closed multilamellar vesicles. On the other hand, PCs in the solid state form extended multilayer sheets. At the solid to fluid transition, multilamellar vesicles appear to “bud off” from the surface. Quasi-elastic light scattering (QELS) measurements indicated that for the PEs, bundle size is independent of acyl chain length n, but that the sizes of vesicles which form at the solid to fluid transition are positively correlated with n. The results of temperature jump experiments showed that once the transition temperature was reached, vesicle formation was largely complete within 30 s.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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