Journal article
The human photoreceptor membrane guanylyl cyclase, RetGC, is present in outer segments and is regulated by calcium and a soluble activator
Neuron (Cambridge, Mass.), v 12(6), pp 1345-1352
01 Jun 1994
PMID: 7912093
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A human photoreceptor membrane guanylyl cyclase, RetGC, was recently cloned and expressed, but its localization and manner of regulation were not defined. We report here that RetGC is detected primarily in outer segments of human photoreceptor cells. Recombinant RetGC can be stimulated by a soluble retinal-specific factor. Ca
2+ interferes with stimulation of RetGC by this factor with a cooperativity coefficient of 1.7 and EC
50 near 200 nM. The Ca
2+ sensitivities of recombinant RetGC and of guanylyl cyclase activity from rod outer segment membranes are very similar. Our findings indicate that RetGC is a photoreceptor-specific guanylyl cyclase which is stimulated by a retinal-specific activator and inhibited by physiologically relevant concentrations of free Ca
2+. The Ca
2+ sensitivity of RetGC may be responsible for some of the previously reported effects of Ca
2+ on light adaptation and recovery of the dark state.
Metrics
Details
- Title
- The human photoreceptor membrane guanylyl cyclase, RetGC, is present in outer segments and is regulated by calcium and a soluble activator
- Creators
- Alexander M. Dizhoor - University of WashingtonDavid G. Lowe - University of WashingtonElena V. Olshevskaya - University of WashingtonRichard P. Laura - University of WashingtonJames B. Hurley - University of Washington
- Publication Details
- Neuron (Cambridge, Mass.), v 12(6), pp 1345-1352
- Publisher
- Elsevier Inc
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Pennsylvania College of Optometry (PCO)
- Web of Science ID
- WOS:A1994NT46300014
- Scopus ID
- 2-s2.0-0028360657
- Other Identifier
- 991022035114504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Neurosciences