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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Total serum glycan analysis is superior to lectin-FLISA for the early detection of hepatocellular carcinoma
Proteomics. Clinical applications, v 7(9-10), pp 690-700
01 Oct 2013
PMID: 23857719
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
PurposeHepatocellular carcinoma (HCC) is a primary cancer of the liver that is predominantly the result of infection with a hepatotropic virus such as hepatitis B virus or hepatitis C virus. As liver cancer is often asymptomatic, the development of sensitive noninvasive biomarkers is needed for early detection and improved survival.
Experimental designWe have previously identified alterations in the N-linked glycosylation of serum proteins with the development of HCC and identified many of the proteins that contained the altered glycosylation. In the current study, we compared the ability of the identified proteins to diagnose HCC with the total serum glycan analysis.
ResultsSurprisingly, glycan analysis of total serum had the greatest ability to distinguish HCC from cirrhosis with an AUROC of 0.851, a sensitivity of 73% at a specificity of 88%. When total glycan sequencing was combined with alpha-fetoprotein (AFP), the sensitivity increased to 95% at a specificity of 90%.
Conclusion and clinical relevanceChanges in glycosylation as detected in whole serum could be used to diagnose HCC with greater sensitivity and specificity than that observed through the analysis of specific protein glycoforms or protein levels. Such an assay could have value in the management of those at risk for the development of HCC.
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Details
- Title
- Total serum glycan analysis is superior to lectin-FLISA for the early detection of hepatocellular carcinoma
- Creators
- Mary Ann Comunale - Drexel UniversityMengjun Wang - Drexel UniversityNikhil Anbarasan - Hepatitis B FoundationLucy Betesh - Drexel UniversityAykan Karabudak - Hepatitis B FoundationEthan Moritz - Hepatitis B FoundationKarthik Devarajan - Fox Chase Cancer CenterJorge Marrero - University of Michigan–Ann ArborTimothy M. Block - Drexel UniversityAnand Mehta - Drexel University
- Publication Details
- Proteomics. Clinical applications, v 7(9-10), pp 690-700
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- R01 CA120206; U01 CA168856 / National Cancer Institute (NCI); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Hepatitis B Foundation R01CA120206 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000325479300011
- Scopus ID
- 2-s2.0-84885938288
- Other Identifier
- 991019168703404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology