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Journal article
Transplanting Neural Progenitor Cells into a Chronic Dorsal Column Lesion Model
Biomedicines, v 10(2), p350
01 Feb 2022
PMID: 35203559
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell transplantation therapy is a promising strategy for spinal cord injury (SCI) repair. Despite advancements in the development of therapeutic strategies in acute and subacute SCI, much less is known about effective strategies for chronic SCI. In previous studies we demonstrated that transplants of neural progenitor cells (NPC) created a permissive environment for axon regeneration and formed a neuronal relay across the injury following an acute dorsal column injury. Here we explored the efficacy of such a strategy in a chronic injury. We tested two preparations of NPCs derived from rat spinal cord at embryonic day 13.5: one prepared using stocks of cultured cells and the other of dissociated cells transplanted without culturing. Transplantation was delayed for 4-, 6and 12-weeks post injury for a chronic injury model. We found that the dissociated NPC transplants survived and proliferated for at least 5 weeks post transplantation, in contrast to the poor survival of transplants prepared from cultured NPC stocks. The dissociated NPC transplants differentiated into neurons expressing excitatory markers, promoted axon regeneration into the injury/transplant site and extended axons from graft-derived neurons into the host. These results support the potential of NPC transplants to form neuronal relays across a chronic SCI, but they also underscore the challenges of achieving efficient cell survival in the environment of a chronic injury.
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Details
- Title
- Transplanting Neural Progenitor Cells into a Chronic Dorsal Column Lesion Model
- Creators
- Kazuo Hayakawa - Drexel UniversityYing Jin - Drexel UniversityJulien Bouyer - Drexel UniversityTheresa M. Connors - Drexel UniversityTakanobu Otsuka - Nagoya City UniversityItzhak Fischer - Drexel University
- Publication Details
- Biomedicines, v 10(2), p350
- Publisher
- Mdpi
- Number of pages
- 18
- Grant note
- R01 NS28785 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000778158600001
- Scopus ID
- 2-s2.0-85124090054
- Other Identifier
- 991019167692704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Medicine, Research & Experimental
- Pharmacology & Pharmacy