Journal article
Transplanting neural progenitors into a complete transection model of spinal cord injury
Journal of neuroscience research, v 92(5), pp 607-618
01 May 2014
PMID: 24452691
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Neural progenitor cell (NPC) transplantation is a promising therapeutic strategy for spinal cord injury (SCI) because of the potential for cell replacement and restoration of connectivity. Our previous studies have shown that transplants of NPC, composed of neuron- and glia-restricted progenitors derived from the embryonic spinal cord, survived well in partial lesion models and generated graft-derived neurons, which could be used to form a functional relay. We have now examined the properties of a similar NPC transplant using a complete transection model in juvenile and adult rats. We found poor survival of grafted cells despite using a variety of lesion methods, matrices, and delays of transplantation. If, instead of cultured progenitor cells, the transplants were composed of segmental or dissociated segments of fetal spinal cord (FSC) derived from similar-staged embryos, grafted cells survived and integrated well with host tissue in juvenile and adult rats. FSC transplants differentiated into neurons and glial cells, including astrocytes and oligodendrocytes. Graft-derived neurons expressed glutaminergic and GABAergic markers. Grafted cells also migrated and extended processes into host tissue. Analysis of axon growth from the host spinal cord showed serotonin-positive fibers and biotinylated dextran amine-traced propriospinal axons growing into the transplants. These results suggest that in treating severe SCI, such as complete transection, NPC grafting faces major challenges related to cell survival and formation of a functional relay. Lessons learned from the efficacy of FSC transplants could be used to develop a therapeutic strategy based on neural progenitor cells for severe SCI. (c) 2014 Wiley Periodicals, Inc.
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Details
- Title
- Transplanting neural progenitors into a complete transection model of spinal cord injury
- Creators
- Carla Christina Medalha - SantosYing Jin - Drexel UniversityTakaya Yamagami - Drexel UniversityChristopher Haas - Drexel UniversityItzhak Fischer - Drexel University
- Publication Details
- Journal of neuroscience research, v 92(5), pp 607-618
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- 0578/11-8 / Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) Craig H. Neilsen Foundation Shriners Hospital for Children Drexel University College of Medicine (for the Spinal Cord Research Center)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000332553400007
- Scopus ID
- 2-s2.0-84895927906
- Other Identifier
- 991019167699904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences