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Unusual phase properties of dilauryl phosphatidylcholine (C12PC)
Journal article   Peer reviewed

Unusual phase properties of dilauryl phosphatidylcholine (C12PC)

Leonard Finegold, Walter A. Shaw and Michael A. Singer
Chemistry and physics of lipids, v 53(2), pp 177-184
1990

Abstract

calorimetry dilauryl phosphatidylcholine liposomes phase properties
Aqueous dispersions of diacyl phosphatidylcholines (C nPC) with linear saturated chains of n ⩾ 13 carbons show only a single sharp thermal main transition peak by differential scanning calorimetry (d.s.c.). By contrast C12PC shows, in addition to a sharp peak (−1.8°C), a broad peak located at 5°C. We show by thin layer chromatography, proton and phosphorus nuclear magnetic resonance, and gas and high pressure liquid chromatography, that the broad peak is indeed an intrinsic property of C12PC and not due to an impurity. In mixtures of C12PC with C13PC, the main peaks suggest ideal mixing of the components, but C12PC/C4PC mixtures do not suggest ideal mixing. Mixtures of C12PC with lauric or myristic acids show an accentuation of the broad peak above about 5 mol% acid. Freeze fracture micrographs of C12PC at temperatures above those of the broad peak show smooth faces only, with no morphological evidence for a non-bilayer phase such as H II. We conclude that the broad peak represents a transition from a new phase, intermediate between L β, and L α, to the fully disordered L α state.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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