Journal article
Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans
Proceedings of the National Academy of Sciences - PNAS, Vol.102(3), pp.779-784
18 Jan 2005
PMCID: PMC545516
PMID: 15642945
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Chronic infection with hepatitis B virus (HBV) is associated with the majority of hepatocellular carcinoma (HCC). The diagnosis of HCC is usually made in the late stages of the disease, when treatment options are limited and prognosis is poor. We therefore have developed a method of glycoproteomic analysis in an attempt to discover serum markers that can assist in the early detection of HBV-induced liver cancer. Briefly, a comparative method for analysis of oligosaccharides released from serum glycoproteins and for recovery and identification of proteins with aberrant glycosylation, as a function of cancer diagnosis, is described. The model we have used is the woodchuck (
Marmota monax
), which shares similarities in the glycosylation pattern associated with liver proteins in human HCC. In this report, we show that woodchucks diagnosed with HCC have dramatically higher levels of serum-associated core α-1,6-linked fucose, as compared with woodchucks without a diagnosis of HCC. The coupling of this methodology with 2D gel proteomics has permitted the identification of several glycoproteins with altered glycosylation as a function of cancer. One such glycoprotein, Golgi Protein 73 (GP73), was found to be elevated and hyperfucosylated in animals with HCC. Further, the study showed GP73 to be elevated in the serum of people with a diagnosis of HCC, providing a validation of our approach. The potential of this technology for biomarker discovery and the implications of increased levels of GP73 in liver cancer are discussed.
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Details
- Title
- Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans
- Creators
- Timothy M Block - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Mary Ann Comunale - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Melissa Lowman - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Laura F Steel - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Patrick R Romano - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Claus Fimmel - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Bud C Tennant - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901W. Thomas London - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Alison A Evans - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Baruch S Blumberg - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Raymond A Dwek - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Tajinder S Mattu - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901Anand S Mehta - Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.102(3), pp.779-784
- Publisher
- National Academy of Sciences
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Epidemiology and Biostatistics
- Identifiers
- 991014878087204721
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- Biochemistry & Molecular Biology