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miR-155 overexpression augments anti-viral CD8+ T cell responses (VIR4P.1007)
Journal article   Peer reviewed

miR-155 overexpression augments anti-viral CD8+ T cell responses (VIR4P.1007)

Jennifer Hope, Christoper Stairiker, Donald Gracias, Erietta Stelekati, Alison Carey, Yvonne Mueller and Peter Katsikis
The Journal of immunology (1950), v 192(1_Supplement), pp 143-143.2
01 May 2014
DOI: https://doi.org/10.4049/jimmunol.192.Supp.143.2

Abstract

Abstract MicroRNAs (miRNAs) are highly conserved, small, single-stranded non-coding RNAs that play an essential role in regulating cellular processes. One miRNA in particular, miR-155, has been well characterized in many immune cell subsets. Recently, our lab has demonstrated an essential role for miR-155 in CD8+ T cell responses. Using murine models of influenza virus and Listeria monocytogenes (LM) infection, we have shown that CD8+T cells deficient in miR-155 demonstrate an intrinsic defect in anti-viral and anti-bacterial responses. Currently, we are investigating and characterizing the effect of miR-155 overexpression in CD8+ T cells. Adoptively transferred miR-155-overexpressing OT-I CD8+ T cells demonstrate a greater than 2-fold increase in the absolute number of anti-viral CD8+ T cells in the lungs of infected animals when compared to control OT-I CD8+ T cells at days 8 and 10 post-influenza infection. Interestingly, a significant number of miR-155 overexpressing OT-I CD8+ T cells demonstrate IFNγ- and TNFα-production and LAMP1-expression ex vivo. Overexpression of miR-155 in CD8+ T cells does not affect memory formation. These data suggest that overexpression of miR-155 augments effector CD8+ T cell responses.

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